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tryple select solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher tryple select solution
    Tryple Select Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tryple+select+solution/Neutralization+solution/pm42268475-41-9-12
    Average 94 stars, based on 1 article reviews
    tryple select solution - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    other:

    Article Title: Specific vulnerability of iPSC-derived motor neurons with TDP-43 gene mutation to oxidative stress.
    Article Snippet: iPSC culture iPSCs were maintained in culture plates coated with iMatrix-511 (Nippi) in Stem Fit medium (Ajinomoto) under feeder-free conditions and passaged every 4–7 days by 0.5× TrypLE Select Solution (CTS TrypLE Select Enzyme; Gibco, 0.5 M EDTA pH 8.0; Life Technologies, D-PBS(-); FUJIFILM WAKO) [51, 52].

    Article Title: Specific vulnerability of iPSC-derived motor neurons with TDP-43 gene mutation to oxidative stress
    Article Snippet: iPSCs were maintained in culture plates coated with iMatrix-511 (Nippi) in Stem Fit medium (Ajinomoto) under feeder-free conditions and passaged every 4–7 days by 0.5× TrypLE Select Solution (CTS TrypLE Select Enzyme; Gibco, 0.5 M EDTA pH 8.0; Life Technologies, D-PBS(-); FUJIFILM WAKO) [ , ].

    Cell Culture:

    Article Title: Generation and purification of ACTH-secreting hPSC-derived pituitary cells for effective transplantation.
    Article Snippet: .. As pretreatment, Ff-hPSC-derived aggregates were cultured in 20 mM Y-27632 for 24 h. The pretreated aggregates were dissociated into single cells by treatment with neuron dissociation solution (Wako) at 37 C for 30 min; repeated washing with DMEM/F-12 (Thermo Fisher Scientific); treatment with DMEM/ F-12 containing 0.2% collagenase type I (Wako) and 0.1% BSA at 37 C for 30 min with gentle stirring; repeated washing with PBS; treatment with a 103 TrypLE Select solution (Thermo Fisher Scientific) containing 0.2 mg/mL DNase I (Roche) at 37 C for 10 min; and gentle separation by pipetting. .. The cells were filtered through a 70-mm cell strainer cap (BD Biosciences) and resuspended in sorting buffer (DMEM/F-12) containing 1 mM EDTA, 1% FBS, and 20 mM Y-27632.

    Gentle:

    Article Title: Generation and purification of ACTH-secreting hPSC-derived pituitary cells for effective transplantation.
    Article Snippet: .. As pretreatment, Ff-hPSC-derived aggregates were cultured in 20 mM Y-27632 for 24 h. The pretreated aggregates were dissociated into single cells by treatment with neuron dissociation solution (Wako) at 37 C for 30 min; repeated washing with DMEM/F-12 (Thermo Fisher Scientific); treatment with DMEM/ F-12 containing 0.2% collagenase type I (Wako) and 0.1% BSA at 37 C for 30 min with gentle stirring; repeated washing with PBS; treatment with a 103 TrypLE Select solution (Thermo Fisher Scientific) containing 0.2 mg/mL DNase I (Roche) at 37 C for 10 min; and gentle separation by pipetting. .. The cells were filtered through a 70-mm cell strainer cap (BD Biosciences) and resuspended in sorting buffer (DMEM/F-12) containing 1 mM EDTA, 1% FBS, and 20 mM Y-27632.

    Article Title: DGAT1 regulates keratinocyte proliferation through the modulation of retinoid homeostasis.
    Article Snippet: Immediately after dissection, skin samples were placed in ice-cold dispase digestion buffer (4 mg/mL in PBS; Gibco, Thermo Fisher Scientific) and incubated overnight at 4 °C. .. The epidermal layer was then separated and incubated in TrypLE Select solution (Gibco, Thermo Fisher Scientific) for 10 min, followed by neutralization with CnT-07 medium (CELLnTEC) and gentle mechanical dissociation to obtain single cells. .. Keratinocytes were pelleted by centrifugation (5 min, 180 g), resuspended in CnT-07 medium (CELLnTEC) supplemented with 10 μM ROCK inhibitor, and cultured at 37 °C in a humidified 5% CO2 incubator.

    Incubation:

    Article Title: DGAT1 regulates keratinocyte proliferation through the modulation of retinoid homeostasis.
    Article Snippet: Immediately after dissection, skin samples were placed in ice-cold dispase digestion buffer (4 mg/mL in PBS; Gibco, Thermo Fisher Scientific) and incubated overnight at 4 °C. .. The epidermal layer was then separated and incubated in TrypLE Select solution (Gibco, Thermo Fisher Scientific) for 10 min, followed by neutralization with CnT-07 medium (CELLnTEC) and gentle mechanical dissociation to obtain single cells. .. Keratinocytes were pelleted by centrifugation (5 min, 180 g), resuspended in CnT-07 medium (CELLnTEC) supplemented with 10 μM ROCK inhibitor, and cultured at 37 °C in a humidified 5% CO2 incubator.

    Neutralization:

    Article Title: DGAT1 regulates keratinocyte proliferation through the modulation of retinoid homeostasis.
    Article Snippet: Immediately after dissection, skin samples were placed in ice-cold dispase digestion buffer (4 mg/mL in PBS; Gibco, Thermo Fisher Scientific) and incubated overnight at 4 °C. .. The epidermal layer was then separated and incubated in TrypLE Select solution (Gibco, Thermo Fisher Scientific) for 10 min, followed by neutralization with CnT-07 medium (CELLnTEC) and gentle mechanical dissociation to obtain single cells. .. Keratinocytes were pelleted by centrifugation (5 min, 180 g), resuspended in CnT-07 medium (CELLnTEC) supplemented with 10 μM ROCK inhibitor, and cultured at 37 °C in a humidified 5% CO2 incubator.

    Centrifugation:

    Article Title: Robust and reproducible human intestinal organoid-derived monolayer model for analyzing drug absorption.
    Article Snippet: .. After centrifugation at 200 × g for 5 min at 4 °C and the removal of supernatant, 2 mL of 2.5 × TrypLE Select solution (mixture of TrypLE select (10 ×) (Thermo Fisher Scientific):PBS ( −) = 1:3) was added to the conical tube, and organoid fragments were digested into single cells in a 37 °C water bath for 5–10 min. Then, 2 mL of basal medium was added to each conical tube, and the mixture was well-pipetted and passed through a 70 μm cell strainer. ..

    Suspension:

    Article Title: Bioactive Properties of Phosphate-Modified Silicon Oxycarbide Protective Coatings: Morphology and Functional Evaluation
    Article Snippet: .. To obtain a cell suspension, the surface of the culture bottle was washed with HyClone phosphate-buffered saline (PBS; Cytiva, USA) after removing the medium, and the cells were separated from the bottom of the bottle with Gibco TrypLe Select solution (Thermo Fisher Scientific, USA). .. A culture medium that neutralizes the digestion action by Gibco TrypLe Select was then added, and the cells were centrifuged.

    Article Title: Characterization of Amnion-Derived Membrane for Clinical Wound Applications
    Article Snippet: .. When cells’ confluency reached 70–80%, cells were subcultured using TrypLE Select solution (Gibco, Grand Island, NY, USA), and a single cell suspension was created and then counted using a hemocytometer and Tyrpan Blue. .. To begin SEM setup, four 10 mm biopsy samples of the membrane were prepared in a 48-well Corning plate, each well filled with 1 mL of DMEM complete media and incubated overnight for acclimation.

    Saline:

    Article Title: Bioactive Properties of Phosphate-Modified Silicon Oxycarbide Protective Coatings: Morphology and Functional Evaluation
    Article Snippet: .. To obtain a cell suspension, the surface of the culture bottle was washed with HyClone phosphate-buffered saline (PBS; Cytiva, USA) after removing the medium, and the cells were separated from the bottom of the bottle with Gibco TrypLe Select solution (Thermo Fisher Scientific, USA). .. A culture medium that neutralizes the digestion action by Gibco TrypLe Select was then added, and the cells were centrifuged.

    Single Cell:

    Article Title: Characterization of Amnion-Derived Membrane for Clinical Wound Applications
    Article Snippet: .. When cells’ confluency reached 70–80%, cells were subcultured using TrypLE Select solution (Gibco, Grand Island, NY, USA), and a single cell suspension was created and then counted using a hemocytometer and Tyrpan Blue. .. To begin SEM setup, four 10 mm biopsy samples of the membrane were prepared in a 48-well Corning plate, each well filled with 1 mL of DMEM complete media and incubated overnight for acclimation.



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